Paul Maddox, Aaron Straight, Peg Coughlin, Timothy J. Mitchison, Edward D. Salmon (2011) CIL:13088, Xenopus [NCBITaxon:262014]. CIL. Dataset

Dual-channel spinning-disk confocal time-lapse movie showing anaphase relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules was performed as described in Maddox et al. (2002, 2003), and with a 100X/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. DNA was visualized by adding Oli-green to the extract (final concentration of 1 µg/ml). Images were acquired at 10-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other. Movie is video 1 from J Cell Biol 2003 162:377-382.

双通道旋转盘共聚焦时间间隔电影展示了中期相对向极性微管流。微管荧光斑点显微镜（FSM）的实验操作参照了Maddox等人的研究（2002年，2003年），使用的是100倍/1.4数值孔径的Plan Apochromat物镜（尼康），并在冷却型CCD相机（型号ER；滨松公司）中进行2X2合并。MetaMorph®软件（通用成像公司）用于控制快门、波长选择、图像采集和存储。通过向提取物中添加 Oli-green（最终浓度为1 µg/ml）来可视化DNA。图像采集间隔为10秒。不同荧光素的连续图像采集间隔为3-15秒，具体取决于实验。不同颜色通道的成对图像间隔不超过0.5秒。该电影是J Cell Biol 2003年第162卷第377-382页中视频1。