Drought tolerance during the reproductive process in wheat (Triticum aestivum)
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https://researchdata.edu.au/drought-tolerance-reproductive-triticum-aestivum/2593305
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资源简介：
An RNA-seq data from two commercial Australian wheat varieties with contrasting reproductive drought tolerance. Under controlled environment growth conditions, flag leaf and spike tissues were harvested from drought-tolerant Halberd and drought-sensitive Cranbrook wheat cultivars during the young microspore (YM) growth stage of pollen development. Poly(A+) mRNA was isolated using a Qiagen Oligotex mRNA kit and an Illumina bcl2fastq 2.17.1.14 pipeline was used to generate the sequence data. Q30 quality scores of >92% were obtained across all samples. RNA-seq reads were checked for quality and trimmed for Illumina adapters using BioKanga ngsqc (https://github.com/csiro-crop-informatics/biokanga).\nLineage: The raw data associated with the publication: "Reproductive Stage Drought Tolerance in Wheat: Importance of Stomatal Conductance and Plant Growth Regulators."
本数据集包含两份澳大利亚商业种植小麦品种的RNA测序（RNA-seq）数据，二者的生殖期耐旱性存在显著差异。在可控环境生长条件下，于花粉发育的小孢子早期（young microspore, YM）生长阶段，分别从耐旱小麦品种Halberd与旱敏感小麦品种Cranbrook的旗叶和穗部组织中采集样本。使用Qiagen Oligotex mRNA试剂盒分离Poly(A+) mRNA，并通过Illumina bcl2fastq 2.17.1.14分析流程生成测序数据。所有样本的Q30质量分数均超过92%。使用BioKanga ngsqc（https://github.com/csiro-crop-informatics/biokanga）对RNA-seq测序读数进行质量评估，并剪除Illumina接头序列。
数据集溯源：本原始数据关联的发表文献为：《小麦生殖期耐旱性：气孔导度与植物生长调节剂的重要性》（Reproductive Stage Drought Tolerance in Wheat: Importance of Stomatal Conductance and Plant Growth Regulators.）
提供机构：
Commonwealth Scientific and Industrial Research Organisation
