RNA-seq analysis of gene expression in male and female mouse liver

Gene expression in adult male and female mouse liver assayed by RNA-seq, as part of a study on chromatin states in male and female mouse and their role in sex-biased liver gene expression (A Sugathan and DJ Waxman (2013) Molec Cell Biol, in press). Total liver RNA was prepared from 12 individual male and 12 individual female mice. Four RNA pools, comprised of RNA isolated from 6 individual male or female livers (2 pooled biological replicates for each sex) were then prepared and used for RNA-seq.本数据集为成年雌雄小鼠肝脏的基因表达情况，通过RNA测序（RNA-seq）检测得到，属于一项探究雌雄小鼠染色质状态及其在性别偏好性肝脏基因表达中调控作用的研究内容（A Sugathan与DJ Waxman，2013年，《分子与细胞生物学（Molec Cell Biol）》，已录用待刊）。研究人员从12只雄性和12只雌性成年小鼠中分别提取总肝脏RNA。随后制备4组RNA混合样本：每组样本由6只同性别个体小鼠的肝脏RNA混合而成，每个性别设置2个混合生物学重复样本，所有混合样本均用于RNA-seq实验。