Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 14/01/2007 to 19/01/2007 (Hobart - Dumont d'Urville)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 14/01/2007 to 19/01/2007 on a trip from Hobart to Dumont D'Urville.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于星盘号研究船（RV L'Astrolabe）科考平台（平台编号：FHZI），采集时段为2007年1月14日至2007年1月19日，航次为从霍巴特前往迪蒙·迪尔维尔站的科考航程。
光系统II（PSII）的最大光化学效率，亦称PSII最大量子产额（Fv/Fm），是浮游植物研究中应用最为广泛的荧光参数之一。该参数表征潜在光化学效率，即光合装置捕获的光能用于光化学反应的概率。已有研究表明，Fv/Fm对水体理化性质变化具有瞬时响应，可作为浮游植物整体健康水平与生理活性的诊断指标。结合光系统II吸收截面面积与叶绿素浓度数据，该参数可用于水体初级生产力的测定（Cheah等，2011，《深海研究》（Deep Sea Research））。
采样用水取自3米深度的海水，通过科考船洁净海水管路持续供应。采用快速重复率（Fast Repetition Rate, FRR）荧光技术，在暗适应状态（暗适应时长不低于15分钟）下以1分钟为间隔持续测定荧光产额；测量所用闪光序列包含两组脉冲：100个亚饱和闪光微脉冲（闪光时长1.1 μs，闪光间隔2.8 μs）以及20个弛豫闪光微脉冲（闪光时长1.1 μs，闪光间隔51.6 μs）。