Yeast cells treated with amiodarone, an antiarrhythmic drug that accumulates in vacuolar membrane

An image gallery of wildtype BY4716 yeast cells treated with 25µM amiodarone for 45 minutes. Amiodarone, a cationic amphipath (fat-soluble weak base), is known to cause phospholipidosis in lab animals chronically treated it. Here, amiodarone-treated yeast cells accumulated the drug in vacuolar membrane, causing cup formation. Bilayer expansion is evident at the concavities as white stretches in the membrane.

本数据集包含经25μM胺碘酮（amiodarone）处理45分钟的野生型BY4716酵母菌细胞的图像图库。胺碘酮作为阳离子两亲性物质（脂溶性弱碱），已被证实可在长期给药的实验动物中引发磷脂沉积症（phospholipidosis）。本实验中，经胺碘酮处理的酵母菌细胞会将该药物蓄积于液泡膜（vacuolar membrane），进而诱导杯状结构形成。在膜的凹陷区域可见双层膜扩张现象，表现为膜上的白色延展带。