Sediment and pore water measurements taken from a fish farming area in Bolinao, Philippines, 2017-06-10 and 2017-06-11

Physicochemical parameters and relative abundance of selected nutrient cycling genes were measured in sediment and pore water samples taken from a fish farming area (Site S3 16◦23.097 N, 119◦55.551 E, depth 12.6m) and a control site (Site S1 16◦22.960 N, 119◦54.723 E, depth 15m) not influenced by fish farming. From each site, five pairs of cores representing five biological replicates were collected. One core from each pair was used for physicochemical analysis while the other cores were used for microbial community analysis. Samples from S1 were collected on 2017-06-10 and samples from S3 were collected the following day. The following parameters were measured from the sediment: grain size distribution, % Carbon (%C), % Nitrogen (%N), % total organic carbon (%Corg), and stable isotope (d13C and d15N) content. The following were measured in the pore water: dissolved organic carbon (DOC), total dissolved nitrogen (TDN), ammonium, phosphate, and silicate. Nitrogen (bacterial ammonia monooxygenase/ amoA and nitrite reductase/ nirK) and sulfur (dissimilatory sulfate reductase/ dsrA and sulfur oxidation/ soxB) cycling genes were quantified and normalized against the abundance of the 16S rRNA gene.

本数据集测定了水产养殖区域（S3站位：16°23.097′N，119°55.551′E，水深12.6m）与未受水产养殖影响的对照站位（S1站位：16°22.960′N，119°54.723′E，水深15m）的沉积物及孔隙水样品的理化参数，以及选定养分循环基因的相对丰度。每个站位采集5组岩芯对，作为5次生物学重复。每组岩芯对中的一根用于理化分析，另一根用于微生物群落分析。S1站位样品采集于2017年6月10日，S3站位样品于次日采集。针对沉积物样品，测定了以下参数：粒度分布、总碳百分含量（%C）、总氮百分含量（%N）、总有机碳百分含量（%Corg），以及稳定同位素（δ¹³C与δ¹⁵N）含量。针对孔隙水样品，测定了以下指标：溶解性有机碳（dissolved organic carbon, DOC）、总溶解性氮（total dissolved nitrogen, TDN）、铵态氮、磷酸盐及硅酸盐。对氮循环相关基因（细菌氨单加氧酶（ammonia monooxygenase, amoA）与亚硝酸还原酶（nitrite reductase, nirK））以及硫循环相关基因（异化硫酸盐还原酶（dissimilatory sulfate reductase, dsrA）与硫氧化酶（sulfur oxidation, soxB））进行了定量，并以16S rRNA基因的丰度作为内参完成归一化。