Malaria culture in four different red blood cell populations

To develop an in vitro culture technique in which malaria parasites from the fluorescent labeled donor RBCs are simultaneously inoculated into cultures containing four different target RBC populations.
Conclusion:
We have developed a three-color flow cytometry-based method, which employs the use of biotin/SA-PE, DDAO and SYBR Green I DNA fluorochromes for simultaneously determining the invasion efficacy of malaria parasites in a mixture of 4 different RBC populations. Our method is simple, has reduced bias, more robust, and requires less manipulation of donor RBCs for culture initiation. Biotinylation of different RBCs therefore offers applications to investigate genotype-phenotype association between malaria parasite and its host RBCs on the susceptibility and resistance to malaria invasion. BD FACSDivaTM CS&T Research Beads