Supporting Information associated with bioRxiv manuscript: "Uncoupled evolution of the Polycomb system and deep origin of non-canonical PRC1"

Dataset S1 Proteome database. Table containing all eukaryotic species information from which sequences are used to construct the phylogenetic trees in SI Fig. S1A-H. Dataset S2 Manually curated HMM of RING1, PCGF, EZH, RBBP, EED, and SUZ12. Dataset S3 Materials that were used for the structural alignment. Includes TM align files, HHpred outputs and pdb files of excised C-terminal ß-hairpins. Dataset S4 Materials and Data that were used for the homology analyses of CBX, PHC, RYBP, and SUZ12. For CBX and RYBP, HMM profiles and HMMsearch outputs are provided. For PHC, SAM and MBT HMM profiles and searches, and newick files of our phylogenetic trees are provided. For SUZ12, HMM profile and multiple sequence alignment are provided. Dataset S5 Alphafold structures of putative sequences of RING and PCGF and the newly identified RYBP orthologs. Dataset S6 Blastoutputs of RING, PCGF, EZH, EED, and RBBP. Dataset S7 Multiple sequence alignments used to build phylogenetic trees and newick files for EZH, EED, RBBP, RING and PCGF. Dataset S8 Fasta sequence files of our orthologous groups of the core subunits of PRC1 and PRC2, and newly identified ncPRC1 accessory subunits.

Dataset S1 蛋白质组数据库：包含用于构建补充信息图S1A-H中系统发育树的所有真核生物物种的序列信息。
Dataset S2 人工整理的RING1、PCGF、EZH、RBBP、EED及SUZ12的隐马尔可夫模型（Hidden Markov Model，HMM）数据集。
Dataset S3 用于结构比对的相关材料，包含TM-align比对文件、HHpred分析输出文件，以及剪切得到的C端β-发夹结构的PDB格式（Protein Data Bank，PDB）文件。
Dataset S4 用于CBX、PHC、RYBP及SUZ12同源性分析的材料与数据。其中针对CBX与RYBP，提供了HMM配置文件及HMMsearch分析结果；针对PHC，提供了SAM与MBT型HMM配置文件及搜索结果，以及本研究构建的系统发育树的Newick格式文件；针对SUZ12，提供了HMM配置文件及多序列比对结果。
Dataset S5 RING与PCGF推定序列以及新鉴定的RYBP同源蛋白的阿尔法折叠（AlphaFold）结构数据集。
Dataset S6 RING、PCGF、EZH、EED及RBBP的基本局部比对搜索工具（Basic Local Alignment Search Tool，BLAST）输出结果数据集。
Dataset S7 用于构建EZH、EED、RBBP、RING及PCGF系统发育树的多序列比对文件及对应Newick格式文件数据集。
Dataset S8 PRC1（多梳抑制复合体1，Polycomb Repressive Complex 1）与PRC2（多梳抑制复合体2，Polycomb Repressive Complex 2）核心亚基，以及新鉴定的ncPRC1（非经典多梳抑制复合体1，non-canonical Polycomb Repressive Complex 1）辅助亚基的同源基因簇Fasta格式序列文件数据集。