Fractioned eDNA samples collected from Mettam's Pool (WA) using 16S teleost fish, 16S crustaceans, 16S bacteria, and 18S eukaryotes (universal) primers.

Sequencing Fastq, LCA, BLAST and ZOTU files for the four metabarcoding assays used in the study, which target teleost fish, crustaceans, bacteria, and eukaryotes (universal). \nLineage: The metabarcoding assay design, amplification and library sequencing were performed as described in Takahashi et al. (2020). Metabarcode sequencing used an Illumina MiSeq platform (V2 chemistry). Fusion PCRs were performed on DNA extracts (with appropriate DNA input determined by qPCR) for each fusion assay with unique multiple identifier (MID) tags.

本研究中所使用的4组元条形码（metabarcoding）实验对应的Fastq测序文件、最低共同祖先（LCA）文件、BLAST文件及ZOTU文件，上述实验分别靶向硬骨鱼、甲壳动物、细菌及通用真核生物。
实验流程：元条形码实验的设计、扩增与文库测序均按照Takahashi等人（2020）的研究方法开展。元条形码测序采用Illumina MiSeq平台（V2化学试剂）。针对每组搭载唯一多重标识符（MID）标签的融合PCR实验，均以DNA提取物为模板进行扩增，其适宜的DNA投入量通过实时定量PCR（qPCR）确定。