Replication Data of the Effects of Green Tea and/or Licorice Aqueous Extracts on Lipids Profile of Dimethylnitrosamine-Intoxicated Male Albino Rats.

A) Body weight gain and relative liver weight 1. Effect of the tested materials on the % of body weight gain Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea, licorice or the mixture of both, all caused significant decreases in the % of body weight gain relative to normal control by 74.48 %, 78.48 % or 67.09 % respectively. The increase in the % of the body weight gain caused by DMN was 93.67 % relative to control. Licorice administration to DMN-treated rats led to more increase in body weight gain by 81.70 % while green tea or the mixture of those extracts caused marked reduction relative to DMN-administered rats by 96.08 % or 126.80 % as shown in table 1. 2. Effect of the tested materials on the % of liver/body weight ratio Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.01). Results indicated that green tea, licorice, DMN or the mixture exerted no significant change relative to normal control. The DMN groups administered with green tea, licorice or the mixture showed no significant changes when compared with DMN control group as shown in table 1. B) Biochemical studies 1. Effect of the tested materials on plasma level of total cholesterol Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). The mixture of the extracts caused a decrease but insignificant when it was compared with normal control while licorice, DMN or green tea caused significant decreases 8.18 %, 4.67 % or 4.67 % respectively. The administration of green tea to DMN-intoxicated rats caused a significant decrease by 5.21 % when compared with DMN control rats, while the mixture or licorice caused significant increase by 8.78 % or 6.84 % respectively as shown in table 2. 2. Effect of the tested materials on plasma level of LDL-cholesterol Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea, licorice, DMN or the mixture of extracts induced significant decreases in plasma level of LDL-cholesterol relative to normal control by 21.45 %, 54.18 %, 30.55 or 21.81 % respectively. Green tea enhanced the effect of DMN by producing more decrease (-33.50 %). On the other hand, licorice or the mixture antagonized the effect of DMN by increasing the level by 32.98 % or 46.01 % respectively as compared to DMN control group as shown in table 2. 3. Effect of the tested materials on plasma level of HDL-cholesterol Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.01). As shown in table 2, green tea or licorice exerted no significant changes in the level of plasma HDL-cholesterol relative to normal control, on contrary to that, the mixture of extracts or DMN produced a significant increase by 8.61 % or 5.04 % respectively. Green tea, licorice or the mixture exerted no significant effect on the rising effect of DMN. 4. Effect of the tested materials on plasma level of total-cholesterol/HDL-cholesterol ratio Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea exerted an insignificant change in that ratio relative to normal control, while licorice, DMN or the mixture caused a significant decrease by 11.39 %, 9.90 % or 8.91 % respectively. Green tea synergisted the lowering effect of DMN by decreasing significantly that ratio by 7.69%, while the mixture attenuated the DMN effect by increasing that ratio significantly by 9.34 % when they were compared with DMN control group. On the other hand, licorice intake to DMN-administered rats caused an insignificant increase as shown in table 3. 5. Effect of the tested materials on plasma level of triglyceride (TG) Table 4 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). As shown in table 3, licorice, DMN, green tea or the mixture increased the plasma level triglyceride significantly relative to normal control by 20.03 %, 4.41 %, 3.41 % or 3.33 %. Green tea, licorice or the mixture intake to DMN-administered animals exerted no significant change when compared to the corresponding DMN control group.

A) 体重增重与相对肝重量
1. 受试材料对体重增重百分比的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达极显著水平（P<0.001）。绿茶、甘草或二者混合物均可使体重增重百分比较正常对照组显著降低，降幅分别为74.48%、78.48%和67.09%。二甲基亚硝胺（DMN）处理组的体重增重百分比较对照组升高93.67%。给DMN处理大鼠给予甘草后，体重增重进一步升高81.70%；而给予绿茶或二者提取物混合物则可使DMN处理组的体重增重显著降低，降幅分别为96.08%和126.80%，具体结果见表1。
2. 受试材料对肝/体重比百分比的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达极显著水平（P<0.01）。结果表明，绿茶、甘草、DMN或提取物混合物与正常对照组相比无显著差异。给予绿茶、甘草或提取物混合物的DMN组与DMN对照组相比无显著变化，具体结果见表1。
B) 生化研究
1. 受试材料对血浆总胆固醇水平的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达极显著水平（P<0.001）。提取物混合物与正常对照组相比虽有降低但无统计学显著性；而甘草、DMN或绿茶可使血浆总胆固醇水平显著降低，降幅分别为8.18%、4.67%和4.67%。给DMN中毒大鼠给予绿茶后，血浆总胆固醇水平较DMN对照组显著降低5.21%；而给予提取物混合物或甘草则使该水平显著升高，增幅分别为8.78%和6.84%，具体结果见表2。
2. 受试材料对血浆低密度脂蛋白胆固醇（LDL-cholesterol）水平的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达极显著水平（P<0.001）。绿茶、甘草、DMN或提取物混合物可使血浆LDL-胆固醇水平较正常对照组显著降低，降幅分别为21.45%、54.18%、30.55%和21.81%。绿茶可增强DMN的作用，使LDL-胆固醇水平进一步降低33.50%；而甘草或提取物混合物则可拮抗DMN的作用，使该水平较DMN对照组分别升高32.98%和46.01%，具体结果见表2。
3. 受试材料对血浆高密度脂蛋白胆固醇（HDL-cholesterol）水平的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达显著水平（P<0.01）。如表2所示，绿茶或甘草与正常对照组相比对血浆HDL-胆固醇水平无显著影响；与之相反，提取物混合物或DMN可使该水平显著升高，增幅分别为8.61%和5.04%。绿茶、甘草或提取物混合物对DMN引起的HDL-胆固醇水平升高无显著影响。
4. 受试材料对血浆总胆固醇/HDL-胆固醇比值的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达极显著水平（P<0.001）。绿茶对该比值与正常对照组相比无显著变化；而甘草、DMN或提取物混合物可使该比值显著降低，降幅分别为11.39%、9.90%和8.91%。与DMN对照组相比，绿茶可协同DMN的降脂作用，使该比值显著降低7.69%；而提取物混合物可减弱DMN的作用，使该比值显著升高9.34%。此外，给DMN处理大鼠给予甘草后，该比值无显著升高，具体结果见表3。
5. 受试材料对血浆甘油三酯（triglyceride, TG）水平的影响
表4显示，组间总体效应经单因素方差分析（one-way ANOVA）检验达极显著水平（P<0.001）。如表3所示，甘草、DMN、绿茶或提取物混合物可使血浆甘油三酯水平较正常对照组显著升高，增幅分别为20.03%、4.41%、3.41%和3.33%。给DMN处理动物给予绿茶、甘草或提取物混合物，与对应的DMN对照组相比无显著变化。