gut metagenome

Sexual maturity samples of R. taihangensis were collected from the Taihangshan Mountain in Huixian City, Henan Province, China, in April 2023, and reared in the laboratory conditions until spawning. Then, the freshly laid eggs were transferred to aquatic containers (45 * 33 * 13 cm) filled with water to a depth of 4 cm, and maintained at a water temperature of 21 +/- 1 C. A photoperiod of 12 hours light to 12 hours dark was maintained throughout the whole experiment. The aerated water used in the experiment had a pH of 8.14 +/- 0.02, conductivity of 300.8 +/- 3.31 uS/cm, and dissolved oxygen levels of 8.18 +/- 0.10 mg/L (mean +/- SD). Tadpoles were fed daily with spirulina dry powder, and water was renewed every other day. The developmental stages of tadpoles were determined according to Gosner. Once the tadpoles reached Gosner stage (Gs) 26-28, they were exposed to TCC.