SWATH-MS Proteomic Profiling of Androgen and Anti-androgen Treated LNCaP and C42B Prostate Cancer Cell lines

The current dataset was generated to explore the proteomic alterations stimulated in AR antagonist/anti-androgen (Bicalutamide and Enzalutamide) treated androgen-dependent cell model (LNCaP) in comparison with androgen-independent/castration-resistant cell model (C4-2B). Cells were seeded in 6-well plates using RPMI-1640+5% FBC and were incubated at 37 °C for 48 h. The medium was replaced with androgen-depleted culture medium containing 5% charcoal-striped serum for 48 h. Next, cells were supplemented with androgens: DHT (10 nM DHT), or anti-androgens: BICALUTAMIDE and ENZALUTAMIDE (10 μM) and Ethanol (EtOH: 20%) and incubated at 37 °C for additional 48 has described previously. C4-2B cells were cultured in RPMI-1640+5% FBS.

The SWATH-MS label-free quantification method was used to obtain the total proteome of LNCaP and C42B cell lines. Current dataset provides the DDA and SWATH mass spectrometry files of androgen/anti-androgen treated LNCaP and C42B cell lines.

本数据集旨在探究经雄激素受体（AR）拮抗剂/抗雄激素（比卡鲁胺（Bicalutamide）与恩扎卢胺（Enzalutamide））处理的雄激素依赖性细胞模型（LNCaP），相较于非雄激素依赖性/去势抵抗性细胞模型（C4-2B）所诱导的蛋白质组学改变。细胞以RPMI-1640培养基添加5% FBC接种于6孔板中，于37 ℃下孵育48 h。随后将培养基更换为含5%活性炭吸附血清的雄激素剥夺培养基，继续孵育48 h。接下来分别向细胞添加雄激素：二氢睾酮（DHT，10 nM），或抗雄激素：比卡鲁胺、恩扎卢胺（浓度均为10 μM）以及乙醇（EtOH：20%），并按前述实验方案于37 ℃下额外孵育48 h。C4-2B细胞采用RPMI-1640培养基添加5% FBS进行培养。
本研究采用SWATH-MS无标记定量方法获取LNCaP与C4-2B细胞系的总蛋白质组。本数据集包含经雄激素/抗雄激素处理的LNCaP与C4-2B细胞系的数据依赖性采集（DDA）质谱文件及SWATH质谱文件。