Cu(II)-based coordination polymers: structural determination and protective effect on myocardial ischemia-reperfusion injury by reducing inflammatory response

In this work, two Cu(II)-containing coordination polymers, namely, {[Cu₂(L)₂(dpyb)(H₂O)₄]·6H₂O}_n (<b>1</b>) and {[Cu₂(L)₂(dpyb)](CH₃CN)₂}_n (<b>2</b>) have been hydrothermally synthesized based on a substituted thiophene carboxylic acid 3,3′-(thiophene-2,5-diyl)dibenzoic acid (H₂L) and auxiliary N-donor co-ligand 1,4-di(pyridin-4-yl)benzene (dpyb). Furthermore, the protective effect of compounds <b>1</b> and <b>2</b> on myocardial ischemia-reperfusion injury (MIRI) was evaluated and the specific was explored. The real-time reverse transcription-polymerase chain reaction (RT-PCR) revealed the down-regulated relative expression of the <i>nf-κb</i> and <i>mapk</i> genes after compound treatment. The enzyme-linked immunosorbent assay (ELISA) suggested the inhibitory of the compound on inflammatory cytokines IL1-β and TNF-α releasing.

本研究以取代噻吩羧酸类配体3,3′-(噻吩-2,5-二基)二苯甲酸（H₂L）与辅助氮供体共配体1,4-二(吡啶-4-基)苯（dpyb）为原料，通过水热法合成了两种含二价铜的配位聚合物，分别为{[Cu₂(L)₂(dpyb)(H₂O)₄]·6H₂O}_n（化合物1）与{[Cu₂(L)₂(dpyb)](CH₃CN)₂}_n（化合物2）。进一步评价了化合物1和2对心肌缺血再灌注损伤（myocardial ischemia-reperfusion injury, MIRI）的保护作用，并探讨了其具体作用机制。实时反转录聚合酶链反应（real-time reverse transcription-polymerase chain reaction, RT-PCR）结果显示，经化合物处理后，nf-κb与mapk基因的相对表达量显著下调。酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）结果表明，此类化合物可抑制炎症细胞因子白细胞介素1β（IL-1β）与肿瘤坏死因子α（TNF-α）的释放。