RNA-seq TPM matrix of oriental tobacco budworm

We collected 4th and 5th instar larvae, female and male pupae, as well as newly emerged male and female adult moths for transcriptome sequencing and gene expression analysis. Subsequently, total RNA was extracted from the aforementioned samples using Trizol reagent (Invitrogen, USA) following the manufacturer's protocol. Illumina RNA sequencing libraries were prepared by Annoroad Gene Technology Company. Clean data was obtained by removing adapters, low-quality reads, and high-content unknown sequences. In addition to our sequencing data, we downloaded 39 transcriptome datasets from the NCBI SRA database which were merged with our dataset. Each sample's data was aligned to the genome using HISAT2 to assess gene transcription levels.