RNA sequencing data of the effect of lactate on BMDMs

The mBMDMs were incubated with 0 and 30 mM lactate for 24 h, respectively. Cells in culture medium without lactate were used as the control group. Total RNA was extracted using TRIzol reagent, and beads with Oligo (dT) were selected for enrichment and purification, followed by fragmentation of the purified mRNA. The fragmented mRNA was then used as a template for the reverse transcription process using random primers to enable the cDNA first strand and second strand synthesis. After that, the library was prepared by end repairing, adding base A, adding sequencing adapter, recovering the target fragment by beads screening, and PCR amplification. Finally, the quality of the library was tested, and the qualified library was sequenced by Illumina platform utilizing the sequencing strategy of PE150.