Decoding transcriptomic signatures of Cysteine String Protein alpha-mediated synapse maintenance

Cysteine string protein a (CSPa), encoded by the Dnajc5 gene, is a synaptic vesicle chaperone that is linked to synapse maintenance and neurodegeneration. To investigate the transcriptional changes associated with synapse maintenance, we performed single nucleus transcriptomics on the cortex of CSPa knockout (KO) mice and littermate controls. Overall design: Nuclei was isolated from CSPa KO and littermate WT mice. DEGs, GO, RNA velocity and CellchatDB analysis were performed for the WT and KO samples.