Additional file 2 of Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit

Supplementary Material 2: Supplementary Fig. 2. Cortical neuronal cultures differentiated from control- and TSC- iPSC lines express the neuronal markers β3-tubulin and MAP2. Cortical neuronal cultures differentiated from control iPSC lines (A) and TSC iPSC lines (B) show a dense network of β3-tubulin- and MAP2-positive neurites. The fluorescence signals of the images in Fig. 2 were enhanced for optimal visualization of the markers; therefore, comparisons of expression levels between the different iPSC lines are not possible from this Figure. We have not observed any obvious and consistent genotype-dependent difference in the level or distribution of β3-tubulin or MAP2 expression. TSP77+/+ and TSP77+/- are isogenic TSC wild type and TSC2 heterozygous mutated iPSC lines, respectively. The neuronal cultures shown here were differentiated between 67-104 days before seeding into the NVUs. Scale bar is 100 µm.