Processed Plaque-2.0 infection scores of the PCL screen for RV, imaged and analysed at UZH.

virus indicates virus genotype, the PCL was tested against, compoundIdentifier indicates the UZH identifier for blinded testing by UZH, PCL_ID and compoundName disclose the PCL compound identifier and name, respectively. Barcode1, Barcode2, Barcode3 and Barcode4 indicate on which screening plates, given by the screening plate sequence number defined by EPFL, the PCL compound was tested on. The PrestoBlue toxicity scoring of the compound tested in noninfected cells is given as 1 (toxic) and 0 (non-toxic) in nonInfectedToxHit. The mean Plaque2.0-based infection scores of the four biological replicates are provided by mean_numberOfNuclei (number of nuclei based on Hoechst staining), mean_numberOfInfectedNuclei (number of GFP reporter-based infected nuclei), mean_infectionIndex (ratio of numberOfInfectedNuclei to numberOfNuclei), mean_numberOfPlaques (number of GFP reporter-based plaques) and mean_totalVirusIntensity (total GFP reporter signal intensity). The infection scores of the positive and negative controls are averaged (mean) over the 32 technical replicates, each, per plate, and the mean PCL compound infection scores were normalized by the mean negative control infection score of the respective plate indicated by by mean_numberOfNucleiRel (number of nuclei based on Hoechst staining), mean_numberOfInfectedNucleiRel (number of GFP reporter-based number of infected nuclei), mean_infectionIndexRel (ratio of numberOfInfectedNuclei to numberOfNuclei), mean_numberOfPlaquesRel (number of GFP reporter-based plaques) and mean_totalVirusIntensityRel (total GFP reporter signal intensity).

virus字段指代本次PCL测试所针对的病毒基因型。compoundIdentifier字段指代苏黎世大学（UZH）用于盲法测试的UZH标识符；PCL_ID与compoundName分别对应披露PCL化合物的标识符与名称。Barcode1、Barcode2、Barcode3与Barcode4字段用于指示该PCL化合物的测试孔板编号，该编号由洛桑联邦理工学院（EPFL）定义的筛选板序列号确定。非感染细胞中测试的化合物的PrestoBlue毒性评分以1（有毒）与0（无毒）的形式记录于nonInfectedToxHit字段中。基于Plaque2.0的四个生物学重复感染评分的平均值通过以下字段提供：mean_numberOfNuclei（基于Hoechst染色的细胞核总数）、mean_numberOfInfectedNuclei（基于绿色荧光蛋白（GFP）报告基因的感染细胞核数）、mean_infectionIndex（感染细胞核数与总细胞核数的比值）、mean_numberOfPlaques（基于GFP报告基因的噬斑数）以及mean_totalVirusIntensity（GFP报告基因总信号强度）。阳性与阴性对照的感染评分基于每块板的32个技术重复取均值；PCL化合物的平均感染评分则以对应板的阴性对照平均感染评分进行归一化，归一化结果由以下字段体现：mean_numberOfNucleiRel（基于Hoechst染色的细胞核总数）、mean_numberOfInfectedNucleiRel（基于GFP报告基因的感染细胞核数）、mean_infectionIndexRel（感染细胞核数与总细胞核数的比值）、mean_numberOfPlaquesRel（基于GFP报告基因的噬斑数）以及mean_totalVirusIntensityRel（GFP报告基因总信号强度）。