Integrated transcriptomic atlas facilitates the understanding of organ development in soybean

Revealing the temporal and spatial expression of each gene is critical for the understanding of plant organ development. To this end, there is increasing demand for the construction of integrated transcriptomic atlases using multiple types of transcriptome data. In this study, we sequenced 315 soybean tissues over the course of the full developmental cycle by bulk RNA-seq and sequenced 5 representative organs (root, nodule, shoot apical meristem, leaf and stem) by snRNA-seq and Stereo-seq. Integrating these transcriptome data, we constructed a comprehensive soybean transcriptomic atlas. Taking the investigations of genes related to blade, vascular bundles, and nodule development as examples, we demonstrate that the integrated transcriptomic atlas has powerful potential for exploring key genes in organ formation. Together, the panoramic soybean transcriptomic atlas provides a valuable resource and will greatly promote functional studies in the future.