Global gene expression of human iPSC directed differentation towards smooth muscle cells using an Acta2eGFP human iPSC reporter line

Here, we generated and differentiated a human induced pluripotent stem cell line with an ACTA2eGFP reporter towards a smooth muscle-like cell that can be purified and expresses characteristic markers of smooth muscle cells. We performed microarray analysis of day 30 ACTA2eGFP+ and ACTA2eGFP- sorted populations, and the transcriptomic profile of the ACTA2eGFP+ cells is reminiscent of an immature or a synthetic smooth muscle cell phenotype. We prepared microarray expression profiles comparing sorted day 30 ACTA2eGFP+ and ACTA2eGFP- candidate SMCs from our iPSC-SMC directed differentiation. Biological triplicates of all samples were prepared. Global gene expression in all 15 samples was analyzed by Affymetrix GeneChip Human Gene 2.0 ST arrays.