PALB2 ChAM acetylation mapping in human cells

The Chromatin Association Motif (ChAM) of the PArtner and Localizer of BRCA2 (PALB2) is essential for DNA repair function by homologous recombination in human cells. Molecular mechanisms governing ChAM interaction with chromatin remain unexplored. In this study, we have mapped acetylated lysines within ChAM domain, which are contained within a region essential to regulate its association with chromatin. GFP-ChAM sample submitted for proteomics analysis for acetylated lysines mapping was purified from HEK293T transiently expressing GFP-ChAM. GFP-ChAM was purified by GFP pull down using GFP-Trap agarose beads (Chromotek) and washed at 250mM NaCl.