AFLP primer combinations and fluorescent dyes.

The first column contains the different MseI-based primers used. The next four columns contain the fluorescently labeled EcoRI-based primers that were used in combination with the MseI-based primer within the same row. The primers are 19 bp in length and consist of a 16 bp core sequence and a 3 bp extension. “m” is short for a GATGAGTCCTGAGTAA core sequence and “e” stands for a GACTGCGTACCAATTC core sequence. “m” and “e” are followed by the three base extensions that differentiate them. The colors of the fluorescent labels of the EcoRI-based primers are presented in the column headers, and the fluorescent 5′ modifications in the cells below them (5-FAM, 6-FAM, JOE, VIC, NED and PET). Individual AFLP markers in Fig. 2 & 3 are characterized by the eNNN-mNNN combinations shown in this table and the PCR product size. The final column describes which fragment analysis instrument was used.