Choroid Plexus Expression Data from Add1 Null Mice on the C57BL/6J Background Strain

Add1 Null mice on the C57BL/6J background develop hydrocephalus that may be due to gene expression changes in the choroid plexus. We used microarrays to detail choroid plexi gene expression prior to the manifestation of hydrocephalus. Choroid plexi from the four ventricle were dissected out, for RNA extraction and hybridization to Affymetrix microarrays, from male Add1 wildtype, heterozygous and null mice at three weeks of age. Five mice were selected for each genotype to assess gene expression prior to the development of hydrocephalus.C57BL/6J遗传背景下的Add1敲除（Add1 Null）小鼠会出现脑积水，其发病机制或与脉络丛（choroid plexus）的基因表达改变相关。本研究采用微阵列（microarrays）技术，在脑积水表型显现前对脉络丛的基因表达谱进行全景式分析。我们选取3周龄的雄性Add1野生型、杂合子及敲除型小鼠，分离其四脑室的脉络丛，用于RNA提取及Affymetrix微阵列杂交实验。每种基因型选取5只小鼠，以评估脑积水发生前的基因表达特征。