Co-evolving JAK2V617F+ relapsed AML and donor T cells with PD-1 blockade after stem cell transplantation: an index case [BCR-seq]

JAK2V617+ myeloproliferative disease has been reported to overexpress PD-L1 rationalizing therapeutic PD-1 blockade. We performed an integrated single cell analysis on samples from an exceptional responder to nivolumab to elucidate determinants of response in this patient. Overall design: Mononuclear cells from peripheral blood were obtained from one patient with transient response to nivolumab and sequenced using 5' scRNA-seq/CITE-seq/scTCR-seq/scBCR-seq on the 10x platform. Gene expression (scRNA-seq) and antibody capture (CITE-seq) are contained in the same feature matrix. scTCR-seq and scBCR-seq are provided individually. Raw reads of gene expression are provided as bam files and raw reads of CITE-seq, scTCR-seq and scBCR-seq as fastq files.