Electrospun polymer nanofibers as solid-phase extraction sorbents for extraction and quantification of microcystins

Electrospun polymer nanofibers were used as novel solid-phase extraction (SPE) sorbents to extract and quantify the microcystins (MCs) including microcystin-RR (MC-RR) and microcystin-LR (MC-LR) from in-suit water samples. The parameters that influenced the extraction efficiency were studied, including the amount of nanofiber, eluted solvent, eluted volume, pH, and the water sample volume. Under optimized conditions, a linear response for MC-RR and MC-LR over the range of 0.25–4 μg/L was achieved with <i>r</i>² values of 0.998 and 0.997, respectively. The extraction recovery of MC-RR and MC-LR was 97%–102% and 98%–100%, respectively, when the MC concentration was 0.25–4 μg/L. When their concentrations ranged from 0.05 μg/L to 0.25 μg/L, the MCs could be detected with high accuracy by the nanofiber SPE sorbent combined with nitrogen gas. Due to its simplicity, environment-friendliness, high efficiency, reusability, and sensitivity, the electrospun polymer nanofiber can be applied as a novel SPE sorbent to extract and detect the MCs from in-suit water samples.Supplemental_materials.zip

本研究采用静电纺丝聚合物纳米纤维（electrospun polymer nanofibers）作为新型固相萃取（solid-phase extraction, SPE）吸附剂，用于从原位水样中萃取并定量检测微囊藻毒素（microcystins, MCs），包括微囊藻毒素-RR（MC-RR）与微囊藻毒素-LR（MC-LR）。研究考察了影响萃取效率的多项参数，包括纳米纤维用量、洗脱溶剂、洗脱体积、pH值以及水样体积。在优化后的实验条件下，MC-RR与MC-LR在0.25~4 μg/L的浓度范围内均呈现良好的线性响应，其决定系数（r²）分别为0.998与0.997。当微囊藻毒素浓度处于0.25~4 μg/L区间时，MC-RR与MC-LR的萃取回收率分别为97%~102%与98%~100%。当二者浓度处于0.05~0.25 μg/L范围时，采用该纳米纤维SPE吸附剂结合氮气检测手段，可高精度检测微囊藻毒素。鉴于该方法具备操作简便、环境友好、高效性、可重复使用性与高灵敏度等优势，静电纺丝聚合物纳米纤维可作为新型SPE吸附剂，用于原位水样中微囊藻毒素的萃取与检测。附带补充材料：Supplemental_materials.zip