Biomass growth and physiological parameters of the macroalga Fucus vesiculosus forma mytili, along with seawater parameters and inorganic nutrient concentrations of the tidal benthic mesocosms on the island of Sylt in spring 2014

Ocean acidification and warming effects on the macroalgal species Fucus vesiculosus forma mytili were simulated in the tidal benthic mesocosm facility at the AWI Wadden Sea Station on the island of Sylt, Germany (55°01'19.2''N, 8°26'17.7''E). The SY1 experiment in spring 2014 (11 weeks from early April to late June) was based on a "Temp X pCO2" full-factorial setup (ambient or delta 5°C temperature X ambient or 1000 ppm pCO2) resulting in 4 treatment levels à 3 replicates.
The seawater parameters (temperature, pH, salinity, total alkalinity, pCO2) and seawater inorganic nutrient concentrations (silicate, nitrite, phosphate, ammonium, total nitrogen oxide, nitrate) were measured on a weekly basis.
The biomass growth rates (RGR) of Fucus vesiculosus forma mytili were calculated over time, and its physiological parameters (carbon, nitrogen, CN ratio, mannitol) were measured at the beginning and end of the experiment.

本研究于德国叙尔特岛（Sylt）阿尔弗雷德·魏格纳研究所（AWI）瓦登海站的潮间底栖中宇宙实验设施内，模拟了海洋酸化与海水增温对大型藻类物种囊岩藻（Fucus vesiculosus forma mytili）的影响，实验点位坐标为55°01'19.2''N、8°26'17.7''E。2014年春季开展的SY1实验周期为11周（4月上旬至6月下旬），采用‘温度×二氧化碳分压（pCO₂）’全因子实验设计，设置4个处理水平：环境温度/增温5℃ × 环境二氧化碳分压/1000ppm二氧化碳分压，各设3个生物学重复。
研究人员每周测定海水参数（温度、pH、盐度、总碱度、二氧化碳分压（pCO₂））与海水无机营养盐浓度（硅酸盐、亚硝酸盐、磷酸盐、铵态氮、总氮氧化物、硝酸盐）。
本研究随实验进程计算了囊岩藻（Fucus vesiculosus forma mytili）的生物量相对生长速率（RGR），并于实验起始与结束阶段测定其生理参数（碳含量、氮含量、碳氮比、甘露醇含量）。