Label-free quantitative proteomics of human healthy gracilis and diseased rotator cuff tear tendons

Rotator cuff tears (RCT) are among the most common causes of shoulder dysfunction, frequently leading to chronic pain and impaired muscle performance. To gain deeper insight into the molecular mechanisms underlying this condition, proteomic approaches offer an unbiased means of characterizing global protein expression changes. In this study, we compared the proteomic profiles of healthy gracilis muscle and rotator cuff tear (RCT) tissue using label-free quantitative proteomics, with the aim of identifying differentially expressed proteins that may serve as biomarkers or provide mechanistic insight into disease progression.