Proteic and phenolics compounds contents in Bacupari callus cultured with glutamine and nitrogen sources

Abstract In this study was evaluated the influence of glutamine supplementation on the endogenous content of amino acids, proteins, total phenolics, flavonoids and proanthocyanidins in Bacupari callus. The explants were inoculated in MS medium, MS with half concentration of the nitrogen salts (MS½) and nitrogen-free MS, supplemented with glutamine (5, 10, 30 and 60mM) named as Gln5, Gln10, Gln30 and Gln60. Amino acids and proteins were analyzed after 20, 80 and 140 days and the secondary metabolites on the 140th day. There was no difference in the amino acids on the 20th day. On the 80th day the treatments MS and MS½ presented the lowest levels. On the 140th day MS and MS½ presented the lowest amino acid concentration and Gln10 the highest. Concerning proteins, there was difference only on the 140th day, being the highest concentrations observed in Gln5, and the lowest in MS½ treatment. Total phenolics content was higher in the treatment Gln60 and lowest in MS. Treatments Gln5, Gln10, Gln30 and MS½ were statistically equal. For flavonoids, the highest values occurred in the treatments Gln30, Gln60 and MS½ and the lowest in Gln5, Gln10 and MS. Similarly, for the proanthocyanidins the highest concentrations were observed in treatment Gln60 and the lowest in Gln5 and MS. In conclusion, the treatment with 60mM of glutamine favors the protein accumulation and production of secondary metabolites in Bacupari callus.

摘要 本研究评估了谷氨酰胺添加处理对巴库帕里愈伤组织（Bacupari callus）内源氨基酸、蛋白质、总酚类、类黄酮及原花青素含量的影响。实验将外植体接种于MS培养基、含半量氮盐的MS培养基（MS½）以及无氮MS培养基中，上述培养基分别添加5、10、30、60mM谷氨酰胺，对应处理组命名为Gln5、Gln10、Gln30与Gln60。分别于接种后20、80、140天检测氨基酸与蛋白质含量，次生代谢物于第140天进行检测。结果显示：第20天时各组氨基酸含量无显著差异；第80天时，MS与MS½处理组氨基酸水平最低；第140天时，MS与MS½处理组氨基酸浓度仍处于最低水平，而Gln10组氨基酸含量最高。关于蛋白质含量，仅在第140天时出现显著差异，其中Gln5组蛋白质浓度最高，MS½组最低。总酚类含量方面，Gln60组总酚含量最高，MS组最低；Gln5、Gln10、Gln30与MS½组之间无统计学差异。类黄酮含量最高的处理组为Gln30、Gln60与MS½，最低的为Gln5、Gln10与MS。原花青素含量的变化趋势与之类似：Gln60组原花青素浓度最高，Gln5与MS组最低。综上，60mM谷氨酰胺处理可促进巴库帕里愈伤组织的蛋白质积累与次生代谢物生成。