Distinctive responses of Mycobacterium smegmatis to exposure of low and high levels of hydrogen peroxide. Distinctive responses of Mycobacterium smegmatis to exposure of low and high levels of hydrogen peroxide

Log phase cultures (OD600 of 0.8 - 1.0) of M. smegmatis mc2155 were diluted 1:100 into 7H9 media and cultured for approximately 12 hours until the OD600 reached 0.3. Re-inoculated cells were then treated with the indicated concentrations of H2O2 (0.2 and 7 mM) for periods of 30 min and the corresponding total RNAs were isolated and compared by RNA-sequencing to RNA from untreated cells that were prepared simultaneously.