Quantitative RT-PCR for neuropeptide and energy sensing genes in hypothalamus.
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Quantitative RT-PCR for selected genes was performed on twelve hypothalamus samples per condition, reactions were done in triplicates. Delta Ct values were determined relative to the Ct value for Ppia in the same sample. P-values were obtained by performing two-tailed Student's t-test on delta Ct values.
创建时间：
2015-12-02
