SIRT6 and SIRT1 contribute to circadian gene expression

Comparison of the hepatic circadian transcriptomes reveals that SIRT6 and SIRT1 separately control transcriptional specificity, and therefore, define distinctly partitioned classes of circadian genes. Livers from WT and SIRT6 KO mice, and livers from WT and SIRT1 KO mice, were harvested over the circadian cycle at ZT 0, 4, 8, 12, 16 and 20 for gene expression analysis.本研究通过比较肝脏昼夜节律转录组（hepatic circadian transcriptomes）发现，沉默信息调控因子6（SIRT6）与沉默信息调控因子1（SIRT1）可分别调控转录特异性，进而划分出两类截然不同的昼夜节律基因亚型。研究人员在昼夜节律周期的昼夜时间点（Zeitgeber Time, ZT）0、4、8、12、16及20，分别采集野生型（Wild Type, WT）与SIRT6基因敲除（Knock Out, KO）小鼠的肝脏，以及野生型（Wild Type, WT）与SIRT1基因敲除（Knock Out, KO）小鼠的肝脏，用于基因表达分析。