Additional file 4 of Pheromone sensing in Drosophila requires support cell-expressed Osiris 8

Additional file 4: Table S1. Analysis of Ir and Or subsystem-enriched genes. Annotations of genes enriched in the Ir (magenta) and Or (green) subsystems, as defined as those showing a >4-fold differential expression between amos and ato mutant antennae. This arbitrary cut-off captures all of the known chemosensory receptors expressed in the two subsystems. The dataset was cleaned from the original microarray data (GSE183763) by updating gene names and removing duplicate gene entries and those corresponding to transposons. Neuronal/non-neuronal expression is a qualitative assessment based upon the Fly Cell Atlas antennal dataset [29] and/or RNA FISH data for chemosensory receptors [17–19]: “1” = expressed; “0” = not expressed; empty cells indicate robust expression was not detected in either cell type (which may reflect sub-threshold expression level). Breadth of expression provides an approximate categorization of cell-type specific and ubiquitously expressed genes within each olfactory subsystem, based upon the qualitative assessment of the number of clusters in the Fly Cell Atlas antennal dataset in which a gene is detected: “1” = 1 cluster; “2” = 2-5 clusters; “3” = >5 clusters.

附加文件4：表S1。Ir与Or亚系统富集基因分析。对Ir（品红色标记）与Or（绿色标记）亚系统中富集的基因进行注释，富集标准定义为：该基因在amos与ato突变体触角间的表达差异倍数大于4倍。该自定义阈值可覆盖两个亚系统中所有已报道的化学感受受体。本数据集源自原始微阵列数据（GSE183763），经更新基因名称、移除重复基因条目及转座子相关基因后完成清洗。神经元/非神经元表达情况为定性评估结果，基于果蝇细胞图谱（Fly Cell Atlas）触角数据集[29]以及化学感受受体的RNA荧光原位杂交（RNA FISH）数据[17–19]得出："1" = 有表达；"0" = 无表达；空白单元格表示未在任一细胞类型中检测到显著表达（这可能反映表达水平低于检测阈值）。表达广度则基于果蝇细胞图谱触角数据集[29]中检测到某基因的细胞簇数量进行定性评估，对每个嗅觉亚系统内的细胞类型特异性基因与泛表达基因进行粗略分类："1" = 仅在1个细胞簇中表达；"2" = 在2-5个细胞簇中表达；"3" = 在5个以上细胞簇中表达。