Identification of candidate FOXA2-regulated genes in the adult mouse uterus

To identify candidate genes regulated by forkhead transcription factor box A2 (FOXA2) in the uterus, control and Foxa2-deleted uteri were collected at day of pseudopregnancy (DOPP) 3.5 (DOPP 0.5= vaginal plug). Microarray analysis identified differentially expressed genes in the Foxa2-deleted as compared to control uteri that are candidiate FOXA2-regulated genes in the uterus. Whole uteri (control wild type n=4; Foxa2-deleted n=4) were analyzed for differences in their transcriptome using a mouse microarray.为鉴定子宫中受叉头框转录因子A2（forkhead transcription factor box A2，FOXA2）调控的候选基因，研究人员于假孕第3.5天（以DOPP计，DOPP 0.5定义为出现阴道栓的当日）收集对照组与Foxa2基因敲除子宫组织。微阵列分析鉴定出Foxa2基因敲除子宫与对照组子宫间的差异表达基因，此类基因即为子宫中受FOXA2调控的候选基因。本次实验采用小鼠微阵列芯片，对完整子宫组织（野生型对照组n=4；Foxa2基因敲除组n=4）的转录组差异进行分析。