Effects of the treatment of osteomodulin on gene expression in cultured human primary trabecular osteoblasts. Effects of the treatment of osteomodulin on gene expression in cultured human primary trabecular osteoblasts

The RNA-seq revealed that only 35 genes (with padj<0.05) were differentially expressed after the OMD 10 ng/ml treatment, and with relatively modest fold-changes to expression. GSEA analysis using webgestalt on GO terms revealed an increase of some genes linked to the response to acid chemicals (genes AKR1C1, AKR1C2, AKR1C3) and a decrease of few genes involved extracellular structure organization (GO:0043062) and ossification (genes ACAN and IBSP), and of the molecular function of actin binding. The Reactome database revealed an up-regulation of genes responsible of collagen network degradation and a down-regulation of ECM proteoglycans, ECM organization, collagen formation, and integrin cell-surface interactions. Overall design: RNA-Seq on primary human OA osteoblasts mRNA cultured 72h in the presence or not of osteomodulin (OMD) 10 ng/mL