scGEX/scTCR-Seq of CD44+ cytotoxic T cells in LCMV-infected Wildtype and Il1rl1-ExAB-/- mice

To study the effect of IL-33 sensing on CD8 T cell differentiation during acute infection with lymphocytic choriomeningitis virus (LCMV), activated CD44+ CTLs were flowcytometrically sorted from infected C57BL/6 wildtype and Il1rl1 gene-targeted mice (Il1rl1-ExAB-/-) and subjected to combined single-cell gene expression and single cell TCR-Seq analysis. Overall design: Comparative gene expression profiling analysis of the effects of IL-33 on CTLs, Th1 and Th2 cells using RNA-Seq and single-cell RNA-Seq of WT- and Il1rl1-ExAB-KO CTLs after an acute LCMV infection.