Effects of blood-feeding on oxygen consumption rates in different metabolic states in <i>A. aegypti</i> FM mitochondria.

Values were expressed as mean ± SEM of nmols oxygen consumed/min/mg protein in four different mitochondrial metabolic states using: pyruvate + proline or Glycerol-3 phosphate, followed by the addition of 1 mM ADP (ADP), 4 µg/mL oligomycin (Oligo) and 5–7 µM FCCP. RCR (respiratory control ratios) were calculated by dividing the rates of oxygen consumption in state 3 (ADP-stimulated) by those of state 4 (in the presence of oligomycin). The inhibition of oxygen consumption promoted by blood feeding were expressed as the percentage relative to their respective metabolic states in SF. SF = sugar fed; BF = 24 h ABM. Statistical analyses between groups were performed by using the Student's t-test. a, p<0.05; b, p<0.0001 both relative to SF.