Killing of B. malayi L3 in rBmHSP12.6 vaccinated mice was evaluated by in vitro (ADCC assay using mouse sera) and in vivo (micropore chamber challenge) assays.

aADCC assay was performed by incubating 50 µl of pooled mice sera (n = 5) samples with 2×105 normal peritoneal exudates cells and 10 B. malayi L3 at 37°C for 48 hrs. Values represent mean ± SD of three wells.bIn vivo micropore chamber assay was performed by surgically implanting 20 B. malayi L3 into the peritoneal cavity of each mouse. 48 hrs after implantation, chambers were removed and larval viability and death determined. Values are mean ± SD. N = 5. Data presented is from one of two similar experiments showing comparable results.*Significant larval death (P