RNA-seq analysis after PIK75 treatment in T-ALL cells

RNA-seq analysis was performed in two T-cell acute lymphoblastic leukemia (T-ALL) cell lines (Jurkat and DND-41) to analyze gene expression changes after the treatment with PIK-75. Overall design: Two T-ALL cell lines (Jurkat and DND-41) were treated with DMSO (control) or PIK-75 (120nM) for 4 hours. Synthesized RNAs (spike-in) was added to the samples based on the cell number. Total RNA was extracted using QIAzol lysis reagent, miRNeasy Mini Kit, and DNase I (Qiagen). Strand-specific library construction and 100-bp paired-end sequencing with a sequencing depth of 20 M reads on the DNBSEQ platform were performed at BGI Genomics.