5-Hydroxymethylcytosine profiling of aging hippocampi

We profiled 5-hydroxymethylcytosine patterns in hippocampi using hydroxymethylated DNA immunoprecipitation followed by Illumina deep sequencing (hMeDIP-Seq). We profiled the hippocampi of 4 young (3 month old) and 4 aged (18 month old) C57BL/6 mice. Additionally, we profiled the 5-hmC patterns of hippocampi that were stereotactically injected with lentiviral constructs that overexpressed Tet2 or luciferase.我们采用羟甲基化DNA免疫沉淀联合Illumina深度测序（hydroxymethylated DNA immunoprecipitation followed by Illumina deep sequencing，简称hMeDIP-Seq），对海马体中的5-羟甲基胞嘧啶（5-hydroxymethylcytosine，简称5-hmC）修饰模式进行了谱型分析。本研究分析了4只3月龄年轻及4只18月龄老年C57BL/6小鼠的海马体。此外，我们还对经立体定向注射过表达Tet2或荧光素酶（luciferase）的慢病毒构建体的海马体的5-hmC修饰模式进行了谱型分析。