Alternative proteins remodel the microbiome and support growth and development in zebrafish

Protein intake is indispensable to growing animals. As populations and resource demands expand, animal protein-based diets cannot be sustained. In order to validate a move completely away from these diets, we fed a model vertebrate alternative proteins during development. Diets were based on pea, milk and whey, as well as fishmeal (control). All diets supported growth to some degree, with the exception of those high in milk and whey proteins. The most promising diets were associated with the upregulation of genes associated with insulin sensitivity and fat storage. The microbiome reflected the dietary changes, with a shift occurring from Fusobacteriota to Proteobacteria. Correlations were also noted with health, as Cetobacterium positively affected development, while Aeromonas did the opposite. Our findings may help direct the selection of specific animal protein-free diets that can be introduced early into development to maximize vertebrate health., , # Alternative proteins remodel the microbiome and support growth and development in zebrafish

Dataset DOI: [10.5061/dryad.v15dv427m](10.5061/dryad.v15dv427m)

## Description of the data and file structure

This file set contains the data required to replicate our analyses in \"alternative proteins support somatic and muscular development while remodeling the microbiome in zebrafish\". This dataset contains survivorship, growth measurements, gene expression values, and gut microbiota data from zebrafish fed various alternative protein diets including pea, milk, and wheat-based formulations over a ~6 month period.

### Files and variables

#### File: Amino_Acid_Data.csv

**Description:** Amino acid profiles of the dietary treatments compared to recommended amounts.

##### Variables

* Diet: Diet treatment group (FM, Fishmeal; P1, Pea-1; P2, Pea-2; M, Milk; M/P2, Milk/Pea-2; W/P2, Wheat/pea-2).
* Percent: Proportion of the specific amino acid within the total protein content of the diet (ex...,