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Comparison of different sample types using different primers and platforms
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2014-06-10
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Comparison of Nanopore and Illumina 16S rRNA gene amplicon sequencing. Nanopore is preferable over Illumina for 16S amplicon sequencing when species-level taxonomic classification, accurate estimation of richness, or focus on rare taxa is required
A mock community and six faecal samples were subjected to 16S rRNA gene amplicon sequencing with Nanopore GridION and Illumina NovaSeq 6000. Each sample was sequenced in three replicates with both seq
NIAID Data Ecosystem80
Amplicon sequences generated (and their corresponding lengths) by each of the primer pairs used in the PCR amplification experiments.
Amplicon sequences generated (and their corresponding lengths) by each of the primer pairs used in the PCR amplification experiments.
NIAID Data Ecosystem50
In-silico analysis for the coverage rate of predicted primer sets.
Primers used in the present and previous studies were submitted to the ‘Probe Match program' facility of the Ribosomal Database Project (http://www.rdp.cme.msu.edu/) to evaluate specificity. *The cove
NIAID Data Ecosystem30
Gut Mycobiome Maturation and Its Determinants during Infancy: A Comparison of Shotgun Metagenomic and ITS2 Amplicon-Based Sequencing Approaches
Samples from subjects recruited as part of the "Microbiome, Antibiotic and Growth Infant Cohort" (MAGIC) study. Fecal samples from enrolled children born at Pennsylvania Hospitals were collected withi
NIAID Data Ecosystem80
Size sorting and primer selection for aquatic metabarcoding
High throughput DNA sequencing of bulk invertebrate samples ('metabarcoding') enables simultaneous identification of multiple taxa for environmental monitoring. The accuracy of metabarcoding studies i
NIAID Data Ecosystem40
