SMART-seq of PD1+ and PD1- CD8 T cells derived from Setx L389S (Setx KI) mice

The goal is to understand the nature of PD-1+ CD8 T cells that are detected in the peripheral blood of ALS4 mice (expressing the mutation SetxL389S, also called Setx KI mice). We performed a SMART (Switching Mechanism At the end of the 5’-end of the RNA Transcript)-sequencing analysis of the transcriptome of effector memory CD8 T cells expressing or not PD-1 from the blood of symptomatic KI animals. Amongst the transcripts differentially expressed between these two populations, we detected upregulation of Tox (Thymocyte Selection Associated High Mobility Group Box) in PD-1+ as compared to PD-1- CD8 T cells. 500 CD44+CD62L-PD1+ and 500 CD44+CD62L-PD-1-CD8 T cells from 10-12 month old KI mice were sorted using a BD FACSAria with 3 or 5 lasers (BD Bioscience). Three individual mice were analyzed.