A transposon mutant library of E. faecalis used to colonize the vaginal tracts of C57BL6/J mice.. Enterococcus faecalis vaginal colonization TnSeq

C57BL6/J mice were vaginally colonized with at mariner-based transposon mutant library of E. faecalis OG1RF. Vaginal swabs used for transposon sequence (TnSeq) analysis were collected 1, 5, and 8 days post colonization. Transposon insertion sites from mutants isolated from vaginal swabs were determined using Illumina sequencing to identify genes involved in E. faecalis vaginal colonization.