TOPBP1 Shapes XY Silencing Dynamics during Meiosis to Ensure Male Fertility

TOPBP1 and other DNA damage response proteins play an important role in meiotic sex chromosome inactivation. We investigate a mutant mouse with a mutation in the BRCT5 domain of TOPBP1 and use single-cell RNA-seq to investigate the effect of the mutation on XY silencing.TOPBP1与其他DNA损伤应答蛋白在减数分裂性染色体失活（meiotic sex chromosome inactivation）过程中发挥重要作用。本研究以携带有TOPBP1的BRCT5结构域突变的突变小鼠为研究对象，采用单细胞RNA测序（single-cell RNA-seq）技术，探究该突变对XY染色体沉默的影响。