Gene expression of human colon cancer HCT116 cells [HOXA5_si]. Gene expression of human colon cancer HCT116 cells [HOXA5_si]

Abberant expression of HOXA5 is implicated in cancer development. We identified a novel long non-coding RNA (HOXA5 short RNA) transcribed from HOXA6-HOX5 locus using a next generation-based target RNA capture system. In this study, HCT116 cells were treated with siRNAs targeting the novel non-coding RNA. Gene expression levels of these cells were measured by microarray analysis. Overall design: HCT116 cells were treated with siRNAs targeting HOXA5 short RNA for 48 h. Total RNA was extracted from these cells, and gene expression was measured by human whole-genome microarray (Agilent Technology).