Back-splice junctions of KSHV circular RNAs
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE165527
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Total RNA of KSHV-infected cells were extracted and RT-PCR was performed using viral circRNA specific divergent primers. Amplicons were sequenced with MiSeq. iSLK-BAC16 and TREx-BCBL1 RTA cell lines were reactivated for lytic infection using Doxicyclin and sodium butyrate for two days followed by RNA extraction and RT-PCR. Amplicons were purified and library preparation was performed followed by deep-sequencing (single-end, 300 nt).
创建时间：
2021-07-20
