Additional file 2: Table S1. of Germ cell and tumor associated piRNAs in the medaka and Xiphophorus melanoma models

Sample sequence distribution. The percentage of clean reads, as supplied by BGI, was between 98.73 and 99.67Â % of total read number. On average 89.25Â % of all clean reads were mapped to the genome. The number of sequences with a length between 25 and 32 bases was on average 3x higher in ovary and testis than in somatic tissues. In contrast to this, the proportion of sequences mapping to known miRNAs from mirBase or non-coding RNA annotated as miRNA from the ncrna dataset from Ensembl for Xiphophorus (Xiphophorus_maculatus.Xipmac4.4.2.ncrna.fa) was significantly higher in somatic cells than in germline cells. In oxidized samples a clear reduction of RNAs other than piRNAs could be observed. (XLSX 14Â kb)

样本序列分布情况。由华大基因（BGI）提供的清洁读数（clean reads）占总读数的比例介于98.73%至99.67%之间。所有清洁读数中，平均有89.25%可比对至基因组。长度介于25至32个碱基的序列数量，在卵巢和睾丸组织中的平均值较体细胞组织高出3倍。与此相反，可比对至miRBase（mirBase）中已知microRNA (miRNA)，或比对至Ensembl数据库中剑尾鱼（Xiphophorus）ncRNA数据集（Xiphophorus_maculatus.Xipmac4.4.2.ncrna.fa）内注释为miRNA的非编码RNA的序列占比，在体细胞中显著高于生殖细胞。在氧化处理样本中，可观察到除Piwi相互作用RNA (piRNAs) 以外的RNA出现明显减少。（XLSX格式，文件大小14 KB）