Extracellular vesicles as prospective biological indicators for midgestational placental complications in the mouse

This study investigates the miRNA content of extracellular vesicles (EVs) isolated from placental tissue at embryonic day 10.5 (D10.5) in a conditional Nodal knockout mouse model that exhibits abnormal placentation. EVs were isolated from enzymatically digested placentae of both Nodal loxP/loxP (control) and Nodal delta/delta (KO) mice. Small RNA sequencing was performed on isolated EVs to identify differentially expressed miRNAs associated with disrupted placental development. Placentae were collected from Nodal loxP/loxP (n=3) and Nodal delta/delta (n=3) embryos at D10.5. EVs were isolated using enzymatic digestion followed by size-exclusion chromatography. Small RNA libraries were prepared from EV RNA and sequenced. Expression profiles were compared between the two groups to identify miRNAs linked to placental dysfunction.本研究针对条件性Nodal基因敲除小鼠模型中胚胎第10.5天（D10.5）的胎盘组织分离得到的细胞外囊泡（extracellular vesicles, EVs）的微小RNA（miRNA）组成展开探究，该模型存在胎盘发育异常表型。研究人员从Nodal loxP/loxP（对照）及Nodal delta/delta（敲除，KO）小鼠的酶解胎盘中分离细胞外囊泡，对分离获得的EVs进行小RNA测序，以鉴定与胎盘发育紊乱相关的差异表达miRNA。研究人员于D10.5天采集了Nodal loxP/loxP组（n=3）与Nodal delta/delta组（n=3）的胚胎胎盘组织。实验先通过酶解结合尺寸排阻色谱法分离细胞外囊泡，随后从EVs的RNA中制备小RNA文库并完成测序。最后对两组的表达谱进行比对分析，以筛选与胎盘功能异常相关的miRNA。