Ribosome profiling uncovers selective mRNA translation associated with eIF2 phosphorylation in erythroid progenitors

The aim of this study is to investigate how eIF2 phosphorylation affects mRNA translation in erythroblasts. Ribosome profiling combined with RNA sequencing was used to determine translation initiation sites and ribosome density on individual transcripts.本研究旨在探究真核翻译起始因子2（eIF2）磷酸化如何影响成红细胞（erythroblasts）中的mRNA翻译过程。本研究采用核糖体谱分析（ribosome profiling）联合RNA测序（RNA sequencing）的方法，对单个转录本上的翻译起始位点与核糖体密度进行测定。