Impact on Glioblastoma U87 Cell Gene Expression of a Carborane Cluster-bearing Amino Acid

Treatment with carborane bearing amino acid on U87 cells showed cytostatic effect followed by cell death. We used microarrays to investigate the effect of the carborane bearing amino acid on U87 cell gene expression profile and explore the mechanism. U87 cells with treatment of 1 mM carborane bearing amino acid and U87 cells without treatment were used for RNA extraction and hybridization on Affymetrix microarrays We performed the treatment 48 h after the cells were cultured to increase the cell population for RNA extraction and the treatments were performed in triplicates to check reproducibility